FastQCFastQC Report
Wed 25 Jan 2023
H3TCYAFX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYAFX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188816695
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCCCAAG18046503595.57684239733145No Hit
AGATCTCGGT33083801.752164976725178Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG5397440.28585607856339185No Hit
AGCTCTCGGT2693980.14267700215809836No Hit
AATTCCAAGG2121100.11233646473898931No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)