FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46494701
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11777492.5330822108093565No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4412180.9489640550651138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT199550.036.7426871
ACCGCAT71200.030.8504961
GATCGTA136600.026.364632
GATCGTT92000.024.2438852
CCGCATA43950.024.2267172
CCGCATT24650.023.2915842
GATCGTC109150.015.9715112
TGAGGTA178750.015.67046452
CCGCATC49150.014.6547642
ATCGTAA104700.014.5198733
ATCGTAT92250.013.5757613
GTGAGGT390550.013.3247231
CGCATAT41800.012.5669163
GATCGTG138900.012.47553352
ATCGTTT86550.010.73179153
ATCGTAC44450.010.4089743
ATCGTAG81200.09.3824473
CCGCATG40900.09.3583952
TGAGGTC141750.08.9353242
ATCGTTA78050.08.7805423
Produced by FastQC (version 0.11.5)