FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677278
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGC1000.0097190917.48973821
CCTAGTG1000.0097190917.48973821
TCAATGC2100.003999474511.66848653
TGTTCAG2852.7416795E-411.06010670
AGTGTTC3207.9783413E-49.85040768
GTGTTCA3400.0013894499.27097169
GGGTTGC3450.00160348889.1264365
AGAGTGT4100.0075360317.689265366
GTCGTGT15250.07.57889270
CGTGTAG9756.1208993E-97.54355970
CGTCGTG17200.07.534161669
GAGTGTT4200.0093649767.504513767
GTAGGGA7904.2156375E-56.650063570
TCGTGTA13401.4351826E-96.534266569
GCGTCGT19000.06.451728368
TAGGGAA8257.66902E-56.368885566
TGTAGGG8751.7229834E-46.004503764
GGGAAAG6450.0095646135.973028768
AGCGTCG20750.05.907167467
GTGTAGG8902.1730432E-45.903303663
Produced by FastQC (version 0.11.5)