FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904177
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA900.00529361219.43323118
CGTGTAT3702.8460956E-49.4682268
GTAGGAT4451.8734824E-48.65873370
AGTGTAG4450.00178635057.871575470
TGTAGGA4955.900091E-47.784113469
GGGAAAG9251.8477294E-87.57373269
TGTAGGG13201.8189894E-127.4302970
GTGTAGG15300.07.32622469
TAGGGAA10603.0589945E-86.939610570
GTAGGGA10754.001231E-86.842778769
CGTGTAG13906.730261E-116.80485768
AGCGTCG27000.06.74623170
TCGTGTA18200.06.737001467
AGAGCGT32850.06.50453370
GTCGTGT19750.06.385654466
CGTCGTG22600.06.354729770
AAGAGCG35500.06.314998669
GAGCGTC28950.06.17082669
AGGGAAA12658.565403E-75.81565768
GGAAAGA13303.5462472E-75.794189570
Produced by FastQC (version 0.11.5)