FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP63_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP63_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753648
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT4652.5273584E-710.54459470
AGAGTGT5251.3303452E-69.33949870
AAGAGTG6755.1868847E-78.3034468
GTAGGGA8352.0545122E-77.55140768
TGTAGGG9352.2564564E-87.491575770
AGGGAAA10909.0221874E-107.390198770
TAGGGAA8553.0626506E-77.37328869
AGCGTCG20050.06.98715570
GTGTAGG10151.01777914E-76.90156866
CGTGTAG10501.8891296E-76.671069665
GCGTCGT18500.06.625994770
GGGAAAG8501.6401522E-56.592586569
CAGATCG8207.065573E-56.40709667
GAGCGTC22000.06.208643469
CGTCGTG16953.092282E-116.19878270
GGAAAGA10751.3138066E-55.86433670
GTCGTGT14101.8202627E-75.712990370
AGAGCGT24300.05.62212168
TCGTGTA12957.9426645E-65.410059568
AAGAGCG26800.05.227679767
Produced by FastQC (version 0.11.5)