FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974026
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACGC850.00378177120.59628163
GCGTAAC950.007225764418.41399627
CGGAAGG2301.2016972E-716.74567270
AAGGGCG1500.00591899814.00547167
GAGGGAA5502.1749781E-47.639347669
GGAGGGA5552.4116976E-47.571306768
CGTGGAG6056.498226E-46.94486267
GTAGGGA6107.133523E-46.888292366
TCGTGGA6000.0045312066.419505666
AGAGCGG6200.0063780666.212104370
TAGGGAA6800.00243833876.178884567
AGAGCGT23900.05.860029769
TGGAGGG7200.00461579355.83561369
TGTAGGG8609.0167846E-45.69990165
GTGTAGG9303.9747366E-45.647367570
AAGAGTG8700.00104438965.63438570
GTCGTGG8750.00112316825.602188665
GGGAAAG8150.0029557995.585292366
GAGCGTC22254.1836756E-115.350404770
CGTCGTG18409.105861E-95.328168470
Produced by FastQC (version 0.11.5)