FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939127
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA4905.2133146E-710.00377370
TAGGGAA11900.08.5326369
AGGGAAA13100.08.01829170
GTAGGGA11455.456968E-127.95272868
AGAGTGT6653.7609661E-67.89771669
TGTAGGG12151.5825208E-107.206294567
AGAGCGT30900.06.798681370
GTGTAGG14201.1277734E-106.65958166
AAGAGCG33850.06.619926569
CGTGTAG13955.16593E-106.527857365
CGTCGTG21150.06.45806668
GTCGTGT18850.06.315379669
AAGAGTG7953.2838783E-46.167568
TCGTGTA17651.4551915E-116.151605664
GCGTCGT23300.06.01085170
GGGAAAG8801.8671283E-45.97007964
GAGCGTC27050.05.82474870
AGCGTCG24950.05.6133469
GAAGAGC38150.05.508139668
CAGATCG11753.134984E-45.065740670
Produced by FastQC (version 0.11.5)