FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP10_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP10_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735998
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGGT1450.004717844514.49192856
GCTACTT1600.00905400413.1333161
ACGTGTT1600.00910319613.122539528
GTGTGTC2852.0150868E-512.28847769
GAGTGTG4602.679146E-48.37658467
TGTAGGG9706.6029315E-107.945344468
TAGGGAA8503.3451215E-87.82848370
GTGTAGG10201.8408173E-97.55379970
AGAGTGT6057.883021E-57.527482566
GTAGGGA8907.532435E-87.47664169
CGTCGTG18850.07.43175970
GCGTCGT19400.07.221064669
AGTGTGT5250.0089361656.672713868
CGTGTAG10752.8947943E-76.51575169
TGTAGCC7601.8599615E-46.44395814
GTCGTGT14702.564775E-106.43264270
AGCGTCG20750.06.41545268
GTAGCCA7802.604185E-46.278728515
GTGTAGC7802.604185E-46.278728513
TCGTGTA12459.737778E-86.190348668
Produced by FastQC (version 0.11.5)