FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_SBP_HALO_bZIP10_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_SBP_HALO_bZIP10_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930021
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2352.7496117E-614.90400670
CGTGCAA1500.00592579814.00295152
ACGGTTC1900.001883534212.90233663
AGAGCGG4156.6781104E-710.97150370
ATGTTCC4753.7970135E-48.11047865
GACTAGA3900.00482034038.08255768
ATTACAG4050.00678093647.7810991
AGGGAAA10755.313268E-97.16778770
GTAGGGA7956.0979746E-67.048938868
TAGGGAA8006.6937137E-67.00488369
TGATCTC5150.00744525166.800489456
CGTGTAG10005.762522E-76.654638370
GGGAAAG10056.2816616E-76.62153168
AAGAGTG7400.0062321795.679634668
AGCGTCG24700.05.6719770
GTCGTGG8800.00120240255.57206670
CGTCGTG22307.2759576E-125.497105670
TCGTGTA11604.4433353E-55.43482369
TGTAGGG10453.2652655E-45.362589468
GGAAGAG43850.05.35150670
Produced by FastQC (version 0.11.5)