FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_pIX_HALO_Nis_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_pIX_HALO_Nis_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172127
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87770.4040739790997488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGA3200.0089168538.74526616
GCTCACA5057.365731E-47.6254631
GCGTCGT31950.07.01446870
AGCGTCG33950.06.704389669
GATACTC6400.0012357736.5639641
AGTATGG7206.727784E-46.3208541
AGAGTGT11752.1839696E-76.25845570
GAGCGTC37300.06.009485768
GTCGTGT26750.05.89080269
GAAGAGC55300.05.7623870
CGTCGTG29950.05.7290869
TCGTGTA25750.05.575608770
AGGGGGG20401.2187229E-105.49142071
AGAGCGT41800.05.445311569
CGTGTAG19451.1223165E-95.401163669
TGTAGGG17601.8155333E-85.37325466
GTTGTCT9200.00212425675.3272751
GTAGGGA15803.1746822E-75.3202367
AAGAGCG45850.05.270792568
CGGAAGA57550.05.172967468
Produced by FastQC (version 0.11.5)