FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_SBP_bZIP53_pIX_HALO_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_SBP_bZIP53_pIX_HALO_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731250
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGC500.007216368327.99386629
GTAGACC950.007197214818.42588654
CACGCAA1550.00739849413.5407644
GTCGTGG4003.144669E-812.25658770
CGGAAGC3000.0051959319.33835270
CGTGTAG8101.2340934E-77.784103468
TGTAGGG7805.6400677E-77.632307570
TTACTAC4150.0084997967.58661217
AGAGTGT4800.00376010737.29558869
GAGATCG8754.490521E-77.20585565
TCGTGTA9653.096775E-76.896321367
GTGTAGG8904.5123506E-66.688988769
GGGAAAG7401.2918534E-46.627006568
AAGAGTG6550.00159571486.41566269
GTCGTGT11701.9911568E-76.286727466
GAGCGTC18751.8189894E-126.16331370
TCGGAAG33200.06.012267669
CAGATCG8751.72394E-46.00446667
AAGAGCG24200.05.788234770
AGAGCGT20655.456968E-125.76581169
Produced by FastQC (version 0.11.5)