FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP9_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP9_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410138
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2900.0039315529.66142460
TGACCGC3800.00384964128.2882232
GTAGGGA45750.06.277214570
TGTAGGG50850.06.26750469
GCGTCAG6250.00701144066.15682277
GTGTAGG58100.05.72725168
CAGTTCG8050.00257347385.652092534
CGCCCAA7450.0067825925.637502736
GAGCGTC116100.05.45997470
TGTTCAG21256.548362E-115.439434568
TAGGGAA48000.05.399266270
GCGTCGT101550.05.311106770
GTCGTGT85450.05.287153270
CGTCGTG96800.05.282284369
AGCGTCG109200.05.259753770
CGTGTAG61550.05.235444567
GTTCAGA21101.6643753E-95.14545169
GTGTTCA21908.240022E-105.117992467
GCCTGAT8950.0089464655.08395842
TCGTGTA76700.05.068411470
Produced by FastQC (version 0.11.5)