FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4377810
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG6650.011.58467670
ACCCCCG2200.005652470511.14187470
AGAGCGG11850.09.45615370
CCAGCCG3700.00300714938.51792162
CGGAAGC14600.08.15471770
GTAGGGA55550.06.6196268
GAGTGTA27900.06.52652569
TGTAGGG65000.06.410862470
TAGGGAA56850.06.34438669
GTGTAGG82250.06.343562670
AGTGTAG26800.06.141096670
TCGTGTT24600.06.12091370
TAGGGTG9751.8655686E-56.106215568
GTAGGGG9751.8685407E-56.105583770
GGGAAAG56400.05.898307369
AGGGAAA69600.05.88653270
GATAGGG14951.6483682E-85.855738670
GGATAGG15302.7343049E-85.72178469
CGTGTAG73000.05.708302570
AGAGTGT35650.05.50118668
Produced by FastQC (version 0.11.5)