FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996585
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG13150.013.04563970
GCGTCGG17400.09.85920370
GAGCGTG13250.08.98375870
CGTCGGG14251.8189894E-127.12578668
AGCGCGG8055.5320997E-56.523646470
GATCGGG29100.06.496744670
CGGAAGC24150.06.378676470
GTCGTGG41000.06.06274870
AGAGCGG32900.05.95918970
AGCGTCG69750.05.772296470
CGTCGTG61600.05.740314569
AGGGCGG10051.8894051E-45.57376970
GCGTCGT63600.05.505445568
CTGATCG7700.0097226875.456140570
CGTGTAG23801.8189894E-125.442767669
GAGCGTC72800.05.3861969
TGGAGGG30350.05.30632970
GTAGGGG16551.5038677E-75.28855570
GTGTAGG29050.05.182237670
GGCGCGG9000.0095160335.057033570
Produced by FastQC (version 0.11.5)