FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP10_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_SBP_bZIP53_HALO_bZIP10_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529313
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG19250.08.00498370
GTAGGCG15600.07.183958570
TCGAGCC13951.0913936E-117.02778638
GAGCCTG12654.0381565E-106.918851440
GACCGCC6300.00103771736.66645233
TGTAGGC30500.06.54505869
GCGTCGG15803.45608E-116.42805270
GTAGGCT19000.06.26705969
AGTGTAG48900.06.23087270
TTCGAGC16902.910383E-116.214061737
CGGAAGC22600.06.19854970
GGGAAAG100600.06.161887670
CCGCCCA8003.603511E-46.1248535
GACGCGC6400.0089559386.01302323
AAGAGTG81050.06.00620670
GCCTGAT15203.8180588E-95.987663742
CAGTTCG14751.2418241E-85.932057434
TAGGCTA20801.8189894E-125.89309170
CGAGCCT17253.1832315E-105.885636339
TGACCGC7750.00163986655.87005532
Produced by FastQC (version 0.11.5)