FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZ_Nis_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZ_Nis_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9886466
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG832390.8419489835902941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG26900.012.4976170
CGGAAGG20200.012.13541670
CGGAAGC49100.09.62853770
AGAGCGG52450.08.81325870
TGTGCCG13450.08.3317470
TCGTGTT55950.07.323080570
GTCGTGG105900.06.87820970
TCGTGTG24550.06.846959670
GATCGGG23900.06.154027570
GTAGGGG51100.06.030728370
GTGTGCC27300.05.772419570
CGTGTAG131700.05.71690169
GTGTAGG140450.05.65994470
TCGTGGG26200.05.61378870
TGTAGGG130350.05.42685770
AGCGTCG308700.05.411152470
GAGCGTG19601.371518E-95.360103670
GAGTGTG72900.05.28412670
CGTCGTG273300.05.227915370
TCGTGTA146150.05.1527468
Produced by FastQC (version 0.11.5)