FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZ_Nis_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZ_Nis_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10611747
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613480.5781140466315301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCG15100.08.11758570
TCGTGTT41300.06.783841670
TGTAGGC51700.06.70626170
CGGAAGC22950.06.25657970
GTCGTGG57400.06.223347770
AGTGTAG84900.06.147533468
CGAGACG6850.00266609366.131299533
GTGTAGG267700.06.04406970
GTAGGCT38000.05.990505769
GCGTCGG11607.342709E-65.73629470
TGTAGGG208900.05.70001870
GAGTGTA82500.05.68835470
TAGGCGA13807.328217E-75.58315170
GTAGGGA183000.05.47329369
TAGGGAA187800.05.445315470
AGAGTGT108100.05.41036570
TAGGCTA44200.05.387910469
CGTGTAG226750.05.282171769
AGAGCGG22854.947651E-105.057821870
Produced by FastQC (version 0.11.5)