FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZ_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZ_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3191264
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTC6350.00113239346.615041741
TAGGGAA42200.06.224042470
TGTAGGG46000.06.16667370
GTAGGGA41450.05.998800369
GTGTAGG49750.05.91302870
CGTGTAG50800.05.72313668
AGAGTGT23800.05.591614769
AAGAGTG30700.05.58960970
GAGTGTT24000.05.544930570
GGGAAAG35600.05.410573569
TCGTGTA66200.05.290957567
GTCGTGT71850.05.166657469
CGTCGTG83300.05.04506669
Produced by FastQC (version 0.11.5)