FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZRI_Nis_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZRI_Nis_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7418494
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362270.4883336159603283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC6208.5867033E-46.778577363
GACGCTT6954.3556016E-46.55206631
AGCCGTG11301.04384526E-76.50903268
TCGTGTT22650.06.49369470
CGCGTCG6800.0024367576.180635564
CCGTGTA6400.0088650656.018986770
GAGCCGT13659.5747964E-85.90073270
GAGTGTT53700.05.803986570
GCCGTGT8507.778008E-45.76792769
AGAGTGT57900.05.624903769
GCGTCGT202750.05.61349370
GTAGGGA100350.05.58358769
AGAGCCG17703.612513E-95.539817369
GTCGTGT164800.05.461181670
TAGGGAA102900.05.44521870
GTGTTCA41600.05.387624770
TGTAGGG108500.05.357831570
CGTCGTG188600.05.291927370
CGTGTAG119200.05.288188570
AGTGTTC41900.05.26547169
Produced by FastQC (version 0.11.5)