FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZRI_Nis_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZRI_Nis_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979432
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG471160.5247102489333401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGT5250.0090186176.6681439
GTCGTGG33400.06.50113870
GTGTAGG198150.06.468910770
AGGGGGG99150.06.39943651
TCGTGTT32400.05.62085770
GAGTGTA68200.05.598466468
CGTGTAG166800.05.585116469
AGTGTAG64150.05.514057669
GTCGTGT228800.05.37273370
CGTCGTG259700.05.36732369
GTAGGGA137500.05.272471469
GCGTCGT276950.05.09718868
TGTAGGG150500.05.09626270
TAGGGAA142150.05.07533370
CGTGTAT28300.05.073896470
GTAGGAT58800.05.06370768
TCGTGTA208200.05.04739768
CGGAAGC17404.3598993E-75.03193470
Produced by FastQC (version 0.11.5)