FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_PpPLATZRI_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_PpPLATZRI_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183321
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG6250.012.32741170
TACGTCC8100.011.67368470
ACGTCCG4054.895446E-711.24132570
GTACGTC8350.010.90475869
GTACGGC2650.001862256210.57239270
CCGGGTG3000.0052018919.33894670
GTGTACG16400.08.32817769
TGTACGT15800.07.537898568
AGTGTAC20250.07.26362670
TGTACGG7451.8718536E-57.051218570
GTAGGGG9854.468875E-76.7553370
ACGTCCT6350.00112370626.619664765
AGAGCGG10602.265133E-76.60774570
GGCAGTT5300.0099372326.60115531
TCGTGTT23550.06.24579370
GAGTGTA27500.06.240114769
CGGAAGC15357.348717E-106.160054770
GTCGTGG29250.06.10623570
TGTAGGG66150.05.876548369
GTAGGGA57450.05.791122469
Produced by FastQC (version 0.11.5)