FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Pool_L2_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Pool_L2_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAGTATCGGACGGTGTAGTATATCTTGAGCAGCATGAGGAAGCCT14.3478260869565215No Hit
CGAGAATCTGGATGTGGGCTGATGCGGCGAGCGNNNNNNNNNNNNNNNNN14.3478260869565215No Hit
GGTATAGCGGGGGTATGATGATGGTGGAGAGAAGAGAATAATGACGTAGT14.3478260869565215No Hit
GGGGGGGGGGCGGGGGGGGGGGGAGGGGGGGGGATGCGGGTGTGGGGAGG14.3478260869565215No Hit
AGAAGTTTCCCGTTTGACTAAAGATAGAAAGTGCATAAACCACATGCCAT14.3478260869565215No Hit
TGATGTGGGGGGGGGGGGGGGGNNNNNNNNNNNNNGGTGGNNNNNNNNGG14.3478260869565215No Hit
AAATCAAAATAAATATTAAATTAATATATTAGCAATCATATTTATATAAT14.3478260869565215No Hit
GGGGGAGGTGGTGGCGGCAGTGTCGGAAGGGCTNNNNNNNNNNNNNNNNN14.3478260869565215No Hit
AAGAGATAAATAATTAGGTTGATGAAAACGAGGTACGAAGATACTATAAG14.3478260869565215No Hit
GAATGCGGCTGGAAGTATGTAGNNNNNNNNNNNNNGGACCNNNNNNNNGC14.3478260869565215No Hit
GAGTCGATGTGATTTTATGCAGAGTTCTGAGCATATATCTTAGTTATATA14.3478260869565215No Hit
GGGGGAGGTCGGGCGGGGGGGGGTGGGCGGCGGGGGGGTAAGAGAGGGAG14.3478260869565215No Hit
CTCTAGGAGTATTCACTTGTAATTATTTTTGTTGTCTTATTCGGTTATAG14.3478260869565215No Hit
AGAAGACCTGACAATAATAGAAAACGGTAGACATGTTTTTTTTATTGGTT14.3478260869565215No Hit
TGCGCGGGGGGATCAAGGGTCCAGCTGGGGTATTGTAGATAGGTTGATAG14.3478260869565215No Hit
CGGCTGGGGCGGAGGGAGATCGGGCGGGGAGGGGGGGGGGGGGGGCTCGG14.3478260869565215No Hit
GGGTGGGCGGCGGCTCGTGGGTGTTGTTCTGATGGCAGTGAACGTTCTTC14.3478260869565215No Hit
AAAAAGAAAACTAAAATAAAGAAAAGTGACAGTATCAAAGCATGGACGTA14.3478260869565215No Hit
TTTTTAGTATTGTTAATTCTTTAGAAGTTTTATCTATTTTACTTTTTTAG14.3478260869565215No Hit
ATATTAAAGTAAAAAACAATATCAATCATTATTATTGATTCTCTATTAGG14.3478260869565215No Hit
AAACCTATCAAAAAAGAAAAATAATTATCAAGAACATCTGAAGCACTGAC14.3478260869565215No Hit
AATAGAACTGGAACTTAAATTCAATGAATTGATAATATATTTTTTTGTCG14.3478260869565215No Hit
CAAATTAAGATATTAAAGAATTGCACAAGTGAATGAGAGCGAGAAGAGCT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)