FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Pool_L1_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Pool_L1_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGCATTCTTTCTACCTATGATACATATCAGCCTGATACCAAAGACA10.7462686567164178No Hit
TAAAAGACCATTTAAATATCAATAGATTAGTCAATCGTGGCCAGAAGGCG10.7462686567164178No Hit
GATATAAAAACACCAACGAAACTGATTCATGTTTACATAAGTAACATCGG10.7462686567164178No Hit
GAGATGTCCCAACGGGCAACTTATCAACACGTCGCAGAAGAGAAAACGCA10.7462686567164178No Hit
CTAGAGGATGATAATAGATTTTTAATCAAGAAATCCTATAGAGCTATCAT10.7462686567164178No Hit
CAAACCTCAACAAGAATAGTAGACCCAAGCAAAAATAAAGTTGTTCTAGT10.7462686567164178No Hit
CCGCCAAAACGATGACGGGGGGTCGGCGGGCGGGGGGAGGAGGTCAGTGG10.7462686567164178No Hit
GATGATGATGCTGATATATAACTTTCAAGGACGACAAGGGGAGGGACGGC10.7462686567164178No Hit
TTTCACAATTTTAACCATTTAAAAATTTGTTGGAAGTTGAATATAAATAT10.7462686567164178No Hit
TATAATTGAAAAAGATTAATACTAAATCTATTACCTTAAACTCCTATACT10.7462686567164178No Hit
GAAANGNNANNNNNNNNNNGNTNNNNANNNNNNNNNNNNNNNNNNCNNNN10.7462686567164178No Hit
GGGGGGGGGGGGGGGGGGGGTGAGGGGGCCTCTGGCAGGCGGGGGCTGGG10.7462686567164178No Hit
CGGCAGGGAGGGGGGGAGGGAGCTCGGGGGCAGGGATTGCAGGGGCAGGG10.7462686567164178No Hit
TAAACAAAGGTAGTCCTAACAATAATGGTGCGTGTGAAAATGGTTAGAAA10.7462686567164178No Hit
AAAAGACATCGGGGCGTCGCGTGCTCTCGAGACCTCTGACGGGGGGGGGT10.7462686567164178No Hit
AGCGGAGAGGAGTGGGATGGTGGGGGAGGGAGTAGGGGAGTGGGGGTAGG10.7462686567164178No Hit
AATATATGAAGATTGGAGGGAGGGAGGAGTAAGGGTGGGTGTACAGCGGG10.7462686567164178No Hit
AAACATTCCACTGAAAGACTCTACTGAGACAAAGATGGGCTGTCAAGAAC10.7462686567164178No Hit
TCAACAAATACCAGAAGCTAGCAAAATAAAATACGACAGACATTAACACA10.7462686567164178No Hit
GCCAGCTCACGCAGCTCACCGATAAGCTCTGCTGGTACCGGCCAGGCACC10.7462686567164178No Hit
AATCTACCGAATTTGCCGCTAGCTTAAATTCACATATCTAACGAGAAAAT10.7462686567164178No Hit
CACTACCAAAGCAAGCTACCCACCCCAAACAACATCGCCGCCCCTACAAC10.7462686567164178No Hit
TCAATTAAAAAAATACTGACAGCTAAGGCGCCTGCGTATGCTGGGGAGAG10.7462686567164178No Hit
TCTCTAAATACGTCGCTGGAAGGAGAGAGAGAGAGGGTGCTACGCTGGAG10.7462686567164178No Hit
CGGCCTAGGAGGGGGGGGGGCGGTATGCGGCTGTGTGGCTGCGGGGGGGC10.7462686567164178No Hit
AATCTTTCAAAAAAAAGAACAAGGTTGTTTAGAGTTTTTTAATCTAATAT10.7462686567164178No Hit
ACACCAATCAAAAAAATATAAAAAATAATAATAAAGTCCAATAATCTTTG10.7462686567164178No Hit
GTTGGTGAGGGGTGGGGGCGGAGCGGGGCGGGGGGGGGGGGAGGAAGGGG10.7462686567164178No Hit
GGAAAAATCGGAGCAGGGAAAATATAAACTCAACAACACCAAAAACAAGT10.7462686567164178No Hit
CACCCCGTAGTCAGACTGTACGTCTCATCCTCATCTAATTCCGCTGATAC10.7462686567164178No Hit
GTACCACAAAGGAGAGGGGGAAGGCGAGGGGCGCGCCTGCTGGGGCGGGG10.7462686567164178No Hit
CNNNNNNGNNNNNGNNNNNNTNNNNNNGNNNNNNNNNNNNNNGNNNNNNN10.7462686567164178No Hit
GATTATAATAAGATTTAATACTCAACAAAAAAAACCATATCAGCATCCAA10.7462686567164178No Hit
ANNNNNNGNNNNNTNNNNNNANNNNNNCNNNNNNNNNNNNNNCNNNNNNC10.7462686567164178No Hit
TGTACGCCCAGCATCAAATGGATAAAATCAATATTATAAATATATTAAAG10.7462686567164178No Hit
AAATATNNNNNNNNNNNNNNNNNNNNTNNNNNNNTNNNNNNNNNNNNNNN10.7462686567164178No Hit
AGACCTAAAATGCACACTAATGAAAAAGAAATTCATAGTAGTCGTTATCT10.7462686567164178No Hit
AGAGTAACCTAACAAACAGATAAAAAAGTGAATAAAAAAGCTGAAAAAGA10.7462686567164178No Hit
TGCTCACTATAGAAATATATAAAAATATAAAAGTAGGAAACATACTACAT10.7462686567164178No Hit
CGTTGCGATGCTTAAAAGGGGGTGGAGGGGAATCGGCGGGTAACTCGTCT10.7462686567164178No Hit
GCCCAAAAAACAATTGGCTGATTCCTCGGACATGCACAGTCACAATCCGG10.7462686567164178No Hit
GGGGACGGCGGGAGGAGGGGCTGGGTGGGGGGGGGGCGGGGGAGGGGGGG10.7462686567164178No Hit
AAAACTTAAATCTCAGAATCTTCTTAATATAAGTATATTAATATTATAAG10.7462686567164178No Hit
ATGATCTTGTCTATGAACAATAGACACTGACAGCAACATTAGATTATAGG10.7462686567164178No Hit
TAAAAGAGAAAAATAAAATATTAATTCGATGTTTGGTTCGTGGTTTTCTG10.7462686567164178No Hit
GTACTAGGAGGGAAGGCTCGGGAGATGAGGGGAGGCGGGAGCGGAGGGGG10.7462686567164178No Hit
CCATACCCGCGAAGATGATCAAAACAATTGTCAAAAAAAAAAATAAAAAA10.7462686567164178No Hit
GCTAAACACTCTTATTAGAAAAATAAAAGTTTTAATTAAAAAAACTTCAA10.7462686567164178No Hit
TTTTTAAATAAATTCAACATAGTTATGATTATATAAAATCAGTTATAAGT10.7462686567164178No Hit
AACCTCAGCAGCAAGATCGTAGGACTAGGGGATGGGGCGGTCGTGAGGGT10.7462686567164178No Hit
CGCATCAATAGCACTGAGGTGTCCGGAGGCACCTCTTATTCTTTGATGAT10.7462686567164178No Hit
NCTCTAATGAAATAATTGTACTATACTTGAAGAATATGTAAACATAAATG10.7462686567164178No Hit
CCCGACAGCTGAGAGTAGCTCTCGGAACGGCATCTACTGAACACATCTAA10.7462686567164178No Hit
TTCAGGGAGCAGGATGCTGGAAGACATCGTGGGAGTGCGGGGGGGGGGGC10.7462686567164178No Hit
AAGTGCTGCATCGTCGAGGAGCATGTGCGTCAGTATCATGGGAAGCGATT10.7462686567164178No Hit
AGAAGTGTACGGTTAGATCGAGGGGGAGTCTCCTCGAGTCGTGCGAGGGT10.7462686567164178No Hit
AATAACCCCTATCATTGTTTCTATGATAAGAAACAATAAAAGCCAAAAAT10.7462686567164178No Hit
GCACTTACCAATCCGACACATCTTATCCATAATCCAACTCATCTGGTCAA10.7462686567164178No Hit
GTTCTAACACGAAATAGACAAAATAACACCAAAAAAACAAGAAATCTAAA10.7462686567164178No Hit
AAGCGTATGCGGGGGAGAGGAGGGGGGGGGGAGGGAGGGCGCAAGGGGGG10.7462686567164178No Hit
AATATACTACCAAGCTCAGGATGCAAGCGAAAAAATAACAGAACGTAAGA10.7462686567164178No Hit
ATAGAGAGGGGAAGGAGGGGGGAGGGGGGGGCGGCGGGGGGCAGAAGGGA10.7462686567164178No Hit
AGTTTCGGCTAACGAGGGAGCGGGGGCGGGGGGGGGAGCTGAGTAGTGAG10.7462686567164178No Hit
GGGTGGGGTAGGGAGGGGGGTGGGGCTGAAGGGAGGCGGGGGGGTGGGGG10.7462686567164178No Hit
CTTATACAATATTTTACTAGTTGCACTCGAACAATAAGGTGAATCTCTAG10.7462686567164178No Hit
GGGGGTGGCTGGGGGGGGGCGGGGGTGGTGGGGGGATGTTGACCGCCGAA10.7462686567164178No Hit
TGACCAGGGGAAATTAGAAAGAGGCAGGATGGTAGGGACGAATTAAAGTA10.7462686567164178No Hit
TGGGAGAATGGACTACAAATCACTAACAATAGATCGTCTAGCTATAAAAA10.7462686567164178No Hit
GACCACTATCTACGCAGCATCAATCAAGGCTTTTCGATCGGATTTACATC10.7462686567164178No Hit
TTTCACTTATATTGGTTAAAGTTTAAAAAAATATTTCAGTATAAAGTATA10.7462686567164178No Hit
GTTGCTCAAGAATGAGTGTATGGGAGGAGGGGGGGTCGTAGGAATAAGGG10.7462686567164178No Hit
AGCGTCAGAAACTAAAGTTAGCACAACAACCGCAAATAAATAACTAATCT10.7462686567164178No Hit
TAAATTGCAGGCGTGTATTTGATTGATTATAACGACTGAAACCGCACCAG10.7462686567164178No Hit
TTTATTGTTTATATCGTCATTCTATGCTATTAATGTTATAAATTGTTATT10.7462686567164178No Hit
GAACAACAAAAAATTAAAGTGCTAAGACTCCGACTCGGAACTCGCCATGT10.7462686567164178No Hit
GTTTGTAATCAGGAAGATGAGGGAAGAACTCGGGGCTGAATGGGCATCGG10.7462686567164178No Hit
AATTACCAAGTACACATAAATAAGATGAAGATGCAAAGTGGGGAAGGTTA10.7462686567164178No Hit
AAGAGACTCAACATCAATGTTATAAAATTGAGCTTCTGGTGACTCCACAA10.7462686567164178No Hit
GGGAGGATGGGGATTGAGAGTGGGATTTGGGAGGGGAAGTAGGGGAGAAA10.7462686567164178No Hit
ATTCTAGGCGGGGAGTATCGAGGAGAGAGGCGGGATCGGGAAGGGAGCTA10.7462686567164178No Hit
AAATGACGTCACACGAAGAGAACACATAATATTGGAATCTATTTTCCCAA10.7462686567164178No Hit
TAGGGGAGAGGGGGGGCGGGGGGTCGGGGGTCGGGCGTCGGTTCCTGGTT10.7462686567164178No Hit
AGCTGGTGTCGGGGAGTTCCACCACCCCCAGTCCCCCTCGCACCAACGCA10.7462686567164178No Hit
AGAAGTGTGGGGGGGGGGGGGGGCTGGGGGGGGGGGGGGGCAGCCGCCGC10.7462686567164178No Hit
GAATCTGTTTTTTTTATGAGCTGAATCTCATTATTTACAAAATTTACTCT10.7462686567164178No Hit
ATAAAGGTAGTTTTGACAGTGTTGGAGCAATCAAATCTTAAAGAAAGAGG10.7462686567164178No Hit
AAAACACAAGCTTCGTAAAAGAAAGGAGGGCCATAACGTCGCCCACATGC10.7462686567164178No Hit
ATACAGGACAAGACTCAACCATATTCGAGTGAGTACTGAGTGGTGCGTGT10.7462686567164178No Hit
GGGCGAACCGTAGGGGGTAGGGGGGAGGGGGGGGGGGGGGGGGGGGGCGG10.7462686567164178No Hit
CGATTATACTAGCGCGTGTCAGATCTGGGGTCGGTGTGGAATTCAGGGAG10.7462686567164178No Hit
CACGAGGGAGGAGGGGGGGGGGGAGGGCTGGGGGGGAGGAAGGGAGGGGG10.7462686567164178No Hit
AGGGGAGGGAGGCGTGGGGAGAGGGGGATCTTCGCAGCGGGCGCGGGGCG10.7462686567164178No Hit
GATTATCATCGTAATAATTTTAGAAGATTTATAAGAAAAAAATACAACGA10.7462686567164178No Hit
ATGGTCGGAAAGTCTCTGTTACTGAGTCACGACGAGGGCAGCACTGCGAG10.7462686567164178No Hit
AGTCACAGATATAGTACAAATCCTGAGCCCGAGCGGGGGGGGGGGGGTGG10.7462686567164178No Hit
GTCCACCTCTCTCCGACCTGGCAATTCTTCGACAGAATGTTGCACTAAGT10.7462686567164178No Hit
ATAAAAACTCTCACATAAAATAGACAATTATTACTTACATCAACATTAAA10.7462686567164178No Hit
GGAATGTGATTTGTTCATGCTTAATTATCACTGCTAAACTTGTGTATGTC10.7462686567164178No Hit
AACTGACCAGCGGCCCACCCCAACCGCGGTGACCCGCGGCGGTCAGCGGG10.7462686567164178No Hit
AGTAAGAAAATCGAAGATAGACGGGCTGGCGTGGGGCGACAACTCGAGAG10.7462686567164178No Hit
ACGGCAAGATAAAAGAATCACATGAAAAAAATAACCTTGCATATTGTATA10.7462686567164178No Hit
TAGAGCAAAGATCTCTCTCTCTACATGACGCGCTCACAAGGCACGATTCA10.7462686567164178No Hit
GCCCTTAGGAATCATAAATAACATCATAATGATAAATGCGAATATACCAT10.7462686567164178No Hit
GAATCATGTACACACAGACTGCGGCCCAACAGGCACCCTAATGCGAAACA10.7462686567164178No Hit
TACGAGAGGTGGTGGCGGGCTGGGGGGCCCTTGACGCTGGTGGACCTGGG10.7462686567164178No Hit
GCGGCAAGAGGGGGGGTCGGGAGGAGGGGGGGCGGGGGGGGGGGGGGGGG10.7462686567164178No Hit
GACCGTGTAGACGAGAGGCGGAGGGCGGGGGGGGAGGAGTGTAGCGAGGG10.7462686567164178No Hit
TGAAGCGTAGTGAGAGTAGTAGGGGGCGGAGGGGGCGGGGGGGCGGGGGG10.7462686567164178No Hit
GCATCCTCTGTAGTACCGCGCCGGCTGGCACAGAGATAGCCGATGCTTAT10.7462686567164178No Hit
AAAATCGTATGATTTCGTCTACACTAAGGATATAAGATGATGTCGTGTAT10.7462686567164178No Hit
TTGATATAGAGGGAGGGGGGGGATGCTACGGGGGGGAGGCTGGGGGTAGT10.7462686567164178No Hit
ACGCCATAGGCAGAGACGACGGGGGGCTGGGGGGGGGATTCGAGTCGAGG10.7462686567164178No Hit
GCCAACAAGGGGCGAGGGCGGGCGTCGCTCGGGGCTGGGCGGGCGAGGGG10.7462686567164178No Hit
GAAAGGAGTGAGAAGGAGTGAGGAAGGGGGGGGAAAGTGTAGAGGAGGGG10.7462686567164178No Hit
TCACGCAAAATAAATCACAGTAAAATACTTTAACTAGTATAATAGAATAG10.7462686567164178No Hit
GGGCAGGTCGGGGTGGGGGGGGGGCTGTCTGGGGAGCGTGGCGGTGGGGG10.7462686567164178No Hit
TCTACTAAGCAAAGTTTTTATCTTAGATTATGAGAAATTGAAGCTGATCG10.7462686567164178No Hit
ACTAACCTAATTTAAGCAAGCCATTCAGTCTCCCCTAAAGAAGAAGTTCA10.7462686567164178No Hit
GACCAAATAAATAAATAGTTACAGATTGAGAATAAAATAGATAAGAAAGC10.7462686567164178No Hit
GAATTGAATCATGAAGTTCTAACAGCGCCTGCGAGGCCTAACTGACACCG10.7462686567164178No Hit
AGAATTATACATTATAAATAAAATAAGTGAGGCAATAAACATTAATTAAG10.7462686567164178No Hit
AATGGCAATCAGATCATCCACTATTACACTCGATCGACGCGGGGGGTGCC10.7462686567164178No Hit
AACACTAATCTTTTATACCATACCGGTCCTAACCGAACAAAGGGCTAGAG10.7462686567164178No Hit
ATTCTTAAGATGTAAAAGATAATCTCAATTATGACAAGAAATCATTACGA10.7462686567164178No Hit
AACGAAGTCGAAGTAGGCGGGGCGGGGGGCGGGGAGGGGGGCGAGAAATA10.7462686567164178No Hit
GGTTCTAGTACTTAGTCTAAAGGATCCAGAAAGGTTCGAGTCATAGTTGA10.7462686567164178No Hit
ATGGTGAGGCCCGATGGTGAGTAGAAACTATTATCTGCAACTTAATATAT10.7462686567164178No Hit
CTGCACGTCGGCGCGGACAGATGTCAGGGGGGAGATGCGTAGCCGGCATC10.7462686567164178No Hit
AAGGTCGGGCTGGGCGGGGGCCAGCGAGGCCAAGAGGTCACACAGAAGGA10.7462686567164178No Hit
TTTTCAACCTTAGCGTGCGACGGCAGGACTGGGGACCCTCGGTGTGAGGA10.7462686567164178No Hit
GAGCCCTAGCGATGTGAGGCGGGGGAGGGGTGGAGGGGGTCGGTGCTGAG10.7462686567164178No Hit
AGCAATGGTGCGGGGGGGGTGGGGATGTGGGGCTGTGGTGGTCTGCGGGG10.7462686567164178No Hit
AATTTATACACACAAAATAAAAAAACATATTAAGTAAATCATATATAAAG10.7462686567164178No Hit
GTTGACTCGCGATGGCTGGGGAGGGTGCTCGGTCGTCTGGTTGTGGTGGG10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers