FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242523
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC750.00183945323.31947914
GGAGTAG4159.0341846E-48.43418933
GTGTAGG15905.456968E-126.607875370
CGTCGTG25650.06.55377670
GCGTCGT27050.06.4735269
CGTGTAG16551.6370905E-116.34835269
GTAGGGA13003.5155608E-86.19615470
GAGCGTC31750.06.177062570
GAGTGTG6250.0069196226.163826569
AGCGTCG29100.05.897609768
GTCGTGT21900.05.757464468
AGAGCGT34900.05.71988369
AAGAGCG38600.05.625709568
TCGTGTA21351.2732926E-115.57723369
GAAGAGC45400.05.476968370
TGTAGGG15006.318769E-75.37043568
Produced by FastQC (version 0.11.5)