FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140114
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG952.789865E-422.095324
AGAGTGT7900.010.19517770
AAGAGTG9951.1223165E-97.742720669
GGGAAAG10655.5479177E-107.564624365
TCGTTCT4200.0093705287.50489860
TAGGGAA12502.222805E-96.724685763
GTAGGGA12004.7506546E-86.421707666
CGTGTAG14802.0372681E-96.15184970
TCGTGTA18457.2759576E-126.073620369
GTCGTGT19751.8189894E-126.029783768
AGCGTCG26650.05.913019770
GGAAAGA16152.415618E-95.855990466
TGTAGGG13253.3077777E-75.815629568
CGTCGTG22401.8189894E-125.629170468
CAGATCG13101.6514823E-65.61360769
GCGTCGT23900.05.5677570
AAAGAGT17008.054485E-95.56294568
GAGCGTC28900.05.452663470
AGGGAAA15552.2676068E-75.406173764
AAGAGCG36100.05.335185570
Produced by FastQC (version 0.11.5)