FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP63_HALO_bZIP9_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP63_HALO_bZIP9_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295060
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA16200.07.34998470
GGATCCA27950.07.02230931
ACCCCGA6450.00136278026.5078216
AGTGTAG31600.06.317065269
GTAGGCT15155.366019E-106.241352669
GGCGCCA7050.00361879825.9657611
GAGTGTA35250.05.960938570
GGCAAGG8852.0053527E-45.94048261
GGCATTG18404.3655746E-115.90496871
AGGCTAT14354.267713E-85.857158769
GTAGGCG10951.781061E-55.75679770
TGTAGGG73300.05.637681570
TGTAGGC23851.8189894E-125.433906668
GTAGGGA66500.05.425056567
TAGGGAA66550.05.42110868
GGCACAG8450.0044911155.39213041
GTGTAGG99800.05.29875769
GCGTCGA10704.5792822E-45.237632868
GGATCCG14752.576724E-65.22762441
GGCAGCA12251.0450706E-45.15003441
Produced by FastQC (version 0.11.5)