FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP63_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP63_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204426
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCTG800.002667340221.87481944
ACCTGAC1500.00595384913.99345623
GCTTGCG1600.00908432513.12763147
GCGTCGG1900.001886647912.90001570
AGTGTGG3854.262843E-49.0946370
GTCGTGG7603.748264E-77.83215270
CGTGTAG13351.8735591E-106.8192770
GTTTACC5150.00749609536.796062
TGGAGGG6300.00102708436.67134663
GTAGGGA9156.942015E-66.50784168
GAGTGTG6150.00586505056.26272669
TAGGGAA10104.817972E-66.24017770
TTGCTTG6250.00696753766.15969133
CGTCGTG23150.06.049991669
GTGTAGG12751.574881E-76.041688470
TCGTGTA14701.1234079E-85.957057568
TGTAGGG11304.6741534E-65.889825367
GCGTCGT23600.05.788442668
GTCGTGT17804.0763553E-95.509943568
GGGAAAG11654.754398E-55.412267
Produced by FastQC (version 0.11.5)