FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP53_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP53_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836727
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGC6553.3117067E-78.55425370
GTAGGCT5057.339096E-47.62789569
AGTGTAG12655.2750693E-117.199548768
GTAGGGA26100.06.976959770
TAGGCTA6552.115081E-46.950330370
GTGTAGG37450.06.545602369
TAGGGAA26350.06.51206769
TGTAGGG30000.06.42014570
TACAAGG6650.00191600116.3136745
GAGTGTA13003.5302946E-86.19566469
AGGGAAA29700.06.131267570
CGTGTAG33650.06.035959270
GCGTCGT57500.05.785742870
AGAGTGT15403.1186573E-85.686456768
AAGAGTG18401.5879777E-95.519294370
AGCGTCG61800.05.496505369
AGAGCGT74550.05.401987670
TCGTGTA44550.05.266601669
GTCGTGT46900.05.153457668
GGGAAAG21958.6947693E-105.106671367
Produced by FastQC (version 0.11.5)