FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878585
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG200.00694060252.52079452
ACCCTGG850.003802743720.57635322
TACGTCC1000.0096346617.51696668
TGACCAC1605.18304E-415.30366222
AGTGTAC3050.00594516759.18712170
AGAGTGT5603.1906056E-68.75647570
GTCGTGT14850.07.78353369
GTACTTT4500.0020133417.77640443
TCGTGTA14650.07.41162470
CGTCGTG16900.07.255548568
AAGAGTG7056.865777E-56.95549869
GCGTCGT17800.06.691107767
AGCGTCG19050.06.619826366
GTAGGGA8601.9411942E-56.517940568
CGTGTAG10451.2294549E-66.36834670
GGGAAAG8308.3579616E-56.329982365
TAGGGAA9306.1101775E-56.02596269
GAGCGTC20950.06.018770765
TCGGAAG33800.06.010361770
CCGCAAC10405.0073155E-55.72276447
Produced by FastQC (version 0.11.5)