FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135048
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTTG2600.001589877110.7737453
AGAGTGT7201.5752448E-78.26771570
GTCGTGT19450.07.5613470
TGTAGGG12554.3655746E-117.254353570
GTAGGGA10856.4410415E-97.1000670
GTGTAGG11907.694325E-107.062092369
AAGAGTG9057.93023E-76.964549569
TAGGGAA10703.6921847E-86.872339770
CGTCGTG21400.06.872339769
CGTGTAG13159.495125E-106.657959568
GCGTCGT22850.06.5903668
AGCGTCG24300.06.34066467
GAGCGTC26500.06.078554666
GGGAAAG9356.639419E-55.992078369
GGAAAGA13857.825711E-75.562141470
TCGTGTA18551.9354047E-95.47447567
AGAGCGT29550.05.451157665
AAGAGCG32700.05.24707769