FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP10_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP10_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725400
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA950.007173188518.43654464
GAGATCC2100.00400022211.66837753
GTGTTAA4753.9271094E-58.84708569
GGGAAAG7705.7352736E-98.64302668
AGAGTGT4703.378291E-48.19837866
AGTGTTA5552.401424E-47.573405368
AAGAGTG5652.948046E-47.439879465
TAGGGAA9452.7497663E-87.41157870
GTAGGGA9654.0234227E-87.25998664
GAGTGTT5400.00148897137.13562567
TGTAGGG10502.557681E-87.003941569
AGGGAAA10401.5817204E-76.73642967
CGTGTAG12057.3341653E-96.6842670
GTGTAGG11552.2280801E-86.670420670
TGATGTC7451.4303131E-46.57540926
GGAAAGA11351.1215889E-76.48076768
CGTCGTG19250.06.185299470
GCGTCGT20300.06.03788169
TCGTGTA16403.445166E-95.766640768
ATTTCTC7900.0020290925.76433765
Produced by FastQC (version 0.11.5)