FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_HALO_bZIP10_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_HALO_bZIP10_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495156
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGAT3208.047489E-49.84276536
GACTGCT3150.0078373228.8838145
GTGTAGG22850.07.664326770
GAGTGTA7552.8201048E-67.423723767
AGTGTAG7201.1499509E-57.299045668
TGTAGGG18900.06.856936570
CGTGTAG21650.06.633094369
GCGTCGT37750.06.494882670
GTAGGGA15902.6011548E-106.16809670
AGCGTCG40150.06.10664669
GGGAAAG14558.989446E-96.01820969
AGAGTGT10651.1330503E-55.91988369
TAGGGAA16759.349606E-105.85508869
TGTAGGA8050.0025468545.656368370
GAGCGTC43400.05.650873768
GTCGTGT32300.05.638856469
TCGTGTA29450.05.58988470
CGTCGTG35100.05.589696468
AGAGCGT48300.05.29449467
AAGAGCG51450.05.107031366
Produced by FastQC (version 0.11.5)