FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_16Y_64x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_16Y_64x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATATGAATTAATAATTCCACTTTACAAATTATTCATCTTTGCCTG11.8181818181818181No Hit
GTGCGGTAAACCTCGGGTCTCTAGAGACAATGTACCTTCTCTTCTTATCA11.8181818181818181No Hit
GGCTCGNNNGNNNNNNNNNNNNNNNNCTNNNGNNCNNNNNNNNNNNACNN11.8181818181818181No Hit
TACTTGATTATGGAGGCAAACGTATAGAGGAGATGCAGGCTAGCTTGAGA11.8181818181818181No Hit
AAATGTTTATTTTTAATCTTTTTTTTAAAAATATCTGTATAAATAAACAG11.8181818181818181No Hit
ATAAGAACAGTGCGTTCCGAGGCGTGACGTGGGAGAGAGGGGATTTCATA11.8181818181818181No Hit
CCATCATAAAATATAATAAATTAGAAATAAATCACAATAAATACTGACTT11.8181818181818181No Hit
TATGCGTGGGCTGTGCCAGCTGGAAGTGGTCAAAAGAAATAAGGACGTCA11.8181818181818181No Hit
GGATCGTTGATCACAACAGAAAAAGAAACTTATTAACTACTTTGATCTCA11.8181818181818181No Hit
GCAACAAGAAGAACGTCTTGCGAGATCGGGCGGACAGCACACCGTGATTC11.8181818181818181No Hit
GGGTATAAAGACACGGATAATAAAAACACAAGCAAATTAAGGGAGCTGCT11.8181818181818181No Hit
GGGAGTGGTGCAGGGGGGAGGGGGAGGGGTCTCTTCGGGAGTGGGGGGGG11.8181818181818181No Hit
GTGCGGTATATCAAAATAAATAATCTGGCTGCTATCGGTGTTATAATTGT11.8181818181818181No Hit
AGACCACCGAACCCGAAGAAAGGGAAAAGTGGAGTTTTTGATCAGAACTT11.8181818181818181No Hit
GATAGGTCTATTAGTATTCATCCCCAAGATATTACCGAAATGGTTACGTT11.8181818181818181No Hit
AACATAGACAATAAAAAGATCTCTATAGAATCCTTGATTTATTTTTTCTT11.8181818181818181No Hit
GGCTAATGTAATAGAAAACCGAAACATAAAAATAAGTACAAAAAAAAAAC11.8181818181818181No Hit
AGGAGGTGCATCAGTGGACAGGAAGATCAAGGCTGGTGGGTGTGGTGGAG11.8181818181818181No Hit
ATGTGCGGGGGAGGAGGGGAGGGAGGCGGGGCGGAGGGGGGATGTGCTGT11.8181818181818181No Hit
GTTTAAAGATTTCTGCAAGACTAAACGTATGACAACAAAATTCATATAAT11.8181818181818181No Hit
GCGCNGNNGNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNCNNNN11.8181818181818181No Hit
TGTCAGNNNTNNNNNNNNNNNNNNNNTTNNNCNNANNNNNNNNNNNCTNN11.8181818181818181No Hit
AAAGAGGGAGGGGCGGGGGGACGGTGAGGGAGTCTGAGGGGGNNNNNNNN11.8181818181818181No Hit
GATTTTTTACTAGTCATAATTACATTCTTTGGCAAGCATAATAAATTTAT11.8181818181818181No Hit
TCCGCTTCAACAGTCATCTTGATGAGATTTCTGATTTAGTTTTTTTCGTT11.8181818181818181No Hit
AGATAATATCTCAACAAAAGCAAAGGACACCAACAACTCTTGGAAAAGCA11.8181818181818181No Hit
GGATGGNNNCNNNNNNNNNNNNNNNNACNNNANNNNNNNNNNNNNNCGNN11.8181818181818181No Hit
CAGAGGGGAAGCAATTTGCATGATAACACTTACGCGGGGCGTGATGATTG11.8181818181818181No Hit
CAGGGGNNNTNNNNNNNNNNNNNNNNTTNNNCNNCNNNNNNNNNNNCCNN11.8181818181818181No Hit
GGGGGGGGTGGTGGGGGGGGGGGGGGGGGGGGTGCGGGGGGGGGGGGGGG11.8181818181818181No Hit
AGACATTGCAATTACTGCACTTCGACGGTATCCATTCGTACTCAGCTGAA11.8181818181818181No Hit
GGGTAAACACCAAAGGCTAGGCAGGCGTAGGGATCGGTAATCATACTAAG11.8181818181818181No Hit
CGGGAAAAAAAGAAAGATAATTATAGACACATTTTAGCTATCTAAAGCAA11.8181818181818181No Hit
GCTCCCATTGGGTGGAGAAGGTGGGTTGTTGTCGGGTGGCGGGGCGCCGG11.8181818181818181No Hit
TAAATACAGTTTATATATCTAATTAAAGTGTATGGTTTTCGTTTTACAAA11.8181818181818181No Hit
GTCGCGAAATCAATGATTAAGGCTTGTAATGTTACGAAGAAGCTGAAAAT11.8181818181818181No Hit
CTCCCCCACACTCCCCAACAAATCATTAAACAACTTACTCCCCAACTCAC11.8181818181818181No Hit
GTGGAGAAAGAAGGAGAGTAATAGGGACAAGAAGGGAGATAGTGGGAAGT11.8181818181818181No Hit
GCATCTGTTATGAATCAGAACTCTAATAAAATTATGGTTTAATGAGTTTA11.8181818181818181No Hit
AAAAACGAGCAGTCCTAGAAGTATGTGTAATGAACAGAAATCAAACAAAA11.8181818181818181No Hit
GTGAAATCATATAACAAAAAAAATACATAAAGAGTATTATTGAAATTTAA11.8181818181818181No Hit
ACGAAACGCGGGGACTGTGGGGGGGGGGGCTGATAGTAGAGGGGCGGTAG11.8181818181818181No Hit
GTAACCATACAATCTTCAATCTCTTATTAATACAATTCAATAAAATATAA11.8181818181818181No Hit
GTCGAAGTGGGGCGTCTCGGGTACGGTCTCGGGCGTTGACCCACGGCCGT11.8181818181818181No Hit
ANNNNNNCNNNNNGNNNNNNCNNNNNNANNNNNNNNNNNNNNANNNNNNA11.8181818181818181No Hit
GGTGGAATAGATTTTATGGAGTGGATATGTATTGTATAGGTTAAAATCTC11.8181818181818181No Hit
GTAAAGTTTGACCAGATCTATAATTCTTTTAATTCTATCTATAGAATTTG11.8181818181818181No Hit
AAGGCGGCAGACTATGGGGGTGGTCTTGATTGCAACTGGGGGTGCAATTG11.8181818181818181No Hit
GGATCGTATGCTTTTATAGGACCTGGTGATAATTTATTTATCAAATTTCA11.8181818181818181No Hit
GGTTGGTCGATACCATCACAGCGAAAAATTTTAATTTGAATGATAAAATA11.8181818181818181No Hit
TTATGGATAACACCATAAGCAAATCTCCCAGATACCACTGAAATGGAGGG11.8181818181818181No Hit
TGGAAATGAGAGTAGAGTAGTTCATGGATGAATGATATCGGTTTGTTCCA11.8181818181818181No Hit
GTGAGGTGAATGGGTTTGAGACACCTAAAAGCAAGGAATAGAGGAGTTAA11.8181818181818181No Hit
AATGTATTACTTCTTAATGGAAAAACAAATGATTCAGAATAATGGCAGGA11.8181818181818181No Hit
ACCGGTTGCTTGTGGACCAAGGTCAATTAAGATACTGCAAATAACATAAT11.8181818181818181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAA50.070.021
CTTTCAG50.070.048
TAGAAAT50.070.022
TAAATTA50.070.017
TAAATCA50.070.028
TAAATAC50.070.038
CTTTAGT50.070.064
TGACTTT50.070.045
GAAATAA50.070.024
AAATCAC50.070.029
AAATTAG50.070.018
ACTGACT50.070.043
ATTAGAA50.070.020
TAAAATA50.070.07
TCAGGCC50.070.051
GTACCCA50.070.069
CACAATA50.070.033
AATCACA50.070.030
TATAATA50.070.012
AAATATA50.070.09
Produced by FastQC (version 0.11.5)