FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_16Y_4x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_16Y_4x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645833
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25360.3926711704109267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT373800.069.843361
GTGAGGT227300.069.67311
TGAGGTA104750.069.043922
GATCGTA143600.068.955832
GATCGTT104950.068.9456942
GATCGTG61800.068.903472
GATCGTC70400.068.587582
TGAGGTC48550.067.817122
ATCGTGA24350.067.387453
GAGGTAA41350.067.35083
GAGGTAT37050.066.951953
ATCGTAC19200.066.875963
ATCGTAA58150.066.845093
TGAGGTG35700.066.842892
TGAGGTT45350.066.5844042
ATCGTAG26550.066.547553
ATCGTAT46100.066.2549743
ATCGTGC8350.066.202713
GAGGTCT17450.065.7633063
GAGGTAG18850.065.70483
Produced by FastQC (version 0.11.5)