FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_16Y_1x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_16Y_1x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653974
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26310.4023095719401688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT365050.069.812931
GTGAGGT244900.069.585471
GATCGTA142100.069.026962
GATCGTT100950.068.912792
GATCGTG64250.068.8980942
TGAGGTA112700.068.807472
GATCGTC64450.068.684292
TGAGGTC51000.067.860322
ATCGTGC8550.067.531693
TGAGGTG39500.067.418232
ATCGTAG27200.067.414323
ATCGTAA57000.067.2861253
TGAGGTT48400.067.167652
GAGGTAA43550.067.0143363
GAGGTAT39150.066.501433
GAGGTCT20050.066.322213
ATCGTGT21800.066.2953953
ATCGTAT46700.065.71623
ATCGTGA25200.065.543743
GAGGTAG22700.065.516833
Produced by FastQC (version 0.11.5)