FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_14Y_64x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_14Y_64x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719589
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29800.4141252854059748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT407950.069.8984151
GTGAGGT255300.069.531611
GATCGTA161450.069.192162
GATCGTT110850.068.762912
GATCGTG71200.068.667062
GATCGTC72900.068.6499942
TGAGGTA118100.068.631062
TGAGGTC53150.068.084672
ATCGTAG30000.067.534963
ATCGTGA28900.067.320653
ATCGTAA63800.067.1872253
ATCGTAT53500.066.975743
ATCGTAC20800.066.9562453
TGAGGTG38650.066.9155352
GAGGTAA48450.066.878813
GAGGTAT38850.066.471683
GAGGTAG22550.065.794663
ATCGTGG13300.065.511733
ATCGTCA27750.065.4448853
ATCGTTA28950.065.391313
Produced by FastQC (version 0.11.5)