FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_14Y_4x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_14Y_4x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080979
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71330.6598648077344703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT447000.069.8200151
ACCGCAT253450.069.5834051
GTGAGGT275500.069.314831
CCGCATA109450.069.060062
GATCGTA174100.068.9219742
CCGCATT56650.068.5665052
GATCGTC84300.068.492872
GATCGTG78400.068.425042
CCGCATG42150.068.326822
GATCGTT120000.068.266732
TGAGGTA130200.067.783362
CCGCATC55050.067.6988452
CGCATAA35900.067.638413
TGAGGTC56050.066.6783142
ATCGTAA69400.066.599623
CGCATAG20700.066.596943
CGCATAT43600.066.205773
TGAGGTG46600.066.157482
ATCGTGA29900.065.647783
ATCGTAC25750.065.629383
Produced by FastQC (version 0.11.5)