FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_Col_14Y_16x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_Col_14Y_16x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863644
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54340.6291944365965607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT363050.069.7268751
GTGAGGT228050.069.511591
ACCGCAT210950.069.483371
CCGCATA85950.069.193362
CCGCATT46150.068.905442
GATCGTT100750.068.6819462
GATCGTA140500.068.6719362
GATCGTC66000.068.323542
TGAGGTA105500.068.275552
CCGCATG32800.068.2601852
TGAGGTC48200.067.8618852
GATCGTG64500.067.525992
CCGCATC50500.067.265012
CGCATAT32200.067.2505953
CGCATAA29050.066.8356553
TGAGGTG35500.066.320542
CGCATAG17200.066.305643
GAGGTAA44100.065.7623443
ATCGTAG25000.065.628763
ATCGTAA56550.065.3888553
Produced by FastQC (version 0.11.5)