FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL9_Col_B_o3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL9_Col_B_o3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465479
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTC305.893114E-446.63872514
CGTCTAC750.001834314123.32692324
TCGTAAT1350.00294207615.55632550
TTTTCCC2700.002173344110.37424856
ATATAGG3050.00597011449.18078143
GTAGGGA6552.5952455E-57.485330669
TGTAGGG7502.5440368E-67.47107370
AGAGCGT18100.07.352402770
TAGGGAA7006.240162E-57.004131370
GTGTAGG8251.0522963E-56.79188569
TCGTGTA11401.7047569E-86.75837269
AAGAGCG20050.06.63733169
GTCGTGT11753.0880983E-86.55918768
CGTGTAG8156.481512E-56.445519470
AGGGAAA7307.8689243E-46.23655569
GAAGAGC23200.05.889003368
CGTCGTG13152.81354E-75.860870467
GGAAAGA7300.00535090835.7568270
GCGTCGT14001.5664591E-75.753393769
GGAAGAG24650.05.40047467
Produced by FastQC (version 0.11.5)