FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163351
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGC800.0026655421.87728557
GCCAGTG1300.002279970816.16532166
CGAATGA3200.0088646948.75091434
CGTGTAT4200.0093696487.50500364
AGAGTGT9301.6387821E-77.154069470
TGTAGGG16400.06.83264170
AAGAGTG10652.459783E-76.57601669
GAGTGTT8308.396625E-56.328425470
GTGTAGG18255.456968E-126.14001769
CGTGTAG19405.456968E-125.956548769
GTAGGGA14801.306762E-85.91610668
TCGTGTA25800.05.837224568
GTGTTAA7250.0049715815.79696868
GAGCGTC39050.05.739068570
GTCGTGT27300.05.644544667
CGTCGTG31100.05.62978970
GGGGGGT7500.0072732765.601069556
AGGGAAA19056.530172E-105.51453370
TGTTAAG8350.00392692865.452972466
TAGGGAA16501.3911449E-75.306108563
Produced by FastQC (version 0.11.5)