FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291048
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG2850.00342117799.82493136
TCGCCTG2900.00393228239.66084170
TGTAGGG35650.07.662279670
CTTCGCC5700.00264759976.75835270
GTGTAGG38600.06.713783769
CGTGTAG40100.06.6380468
GAGTGTC10803.1733362E-76.485286769
GTCTTCG8802.745002E-56.367372569
TCTTCGC8257.7294695E-56.36737270
GTAGGGA31150.06.296520768
CGAGATC8701.6038166E-46.038025470
CGTCGTG63450.06.01613870
GCGTCGT68200.05.853874769
AGTGTCT12608.0517566E-75.83675870
GTCGTGT55750.05.779175869
TCGTGTA52800.05.770431570
TAGGGAA33100.05.713321769
AGCGTCG72900.05.525110268
AGAGTGT19258.58563E-105.45774870
AGGGAAA37950.05.444565370
Produced by FastQC (version 0.11.5)