FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL16_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL16_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395247
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATG2000.002793760312.24364927
ACGGTGC2050.003362300611.94452222
GTTTCGC3800.0038319488.29213837
AAGAGTG25600.07.25101370
AGAGTGT21600.06.972322570
GAGTGTC12351.720764E-96.806236369
GTAGGGA39150.06.62007270
GGGAAAG32450.06.583819469
TCGTGTT11753.1528543E-86.5576470
TGTAGGG42200.06.307596769
GTCGTGG6700.00206154286.272911570
TAGGGAA39100.06.002649368
GGAAAGA41950.05.9277470
GCGTCGT83700.05.774526670
AGGGAAA44600.05.6540669
GTGTAGG47100.05.651392570
AGCGTCG91300.05.639093470
GTCTTCG9505.2902516E-45.53006769
GTCGTGT70300.05.48024769
CGTGTAG50000.05.463706569
Produced by FastQC (version 0.11.5)