FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL13_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL13_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431176
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG2900.00395934949.65236629
GTGTTCA7301.7036491E-67.677097367
AGTGTTC7402.0891803E-67.573619468
GTTCAGA7151.0479964E-57.34723669
TGTAGGG25200.06.94877670
GTGTAGG28000.06.62913270
TTCAGAG8501.6462394E-56.592344370
TGTTCAG7501.5429423E-46.538557568
CGTGTAG30850.06.357284570
AGAGTGT13359.160431E-96.296058770
GAGTGTT12257.141898E-86.29074165
GTGGGGG6900.00286114456.09182466
AGTGTTA8053.8639744E-46.090752670
TAGGGAA22450.06.083969670
GTAGGGA22500.06.0704570
GGGAAAG17456.730261E-116.02094470
TCGTGTA38650.05.981493568
GAGACTT6450.00962938655.970194340
AAGAGTG14303.9286533E-85.87882368
AGGGAAA24100.05.812751370
Produced by FastQC (version 0.11.5)