FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL13_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL13_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061332
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG1600.00913949413.116238
GGATAGG5957.642193E-78.8290570
GTCGTGG6452.616307E-78.68760370
GATAGGG5301.5169451E-58.59027870
TAGGGAA31200.07.296229470
GTAGGGA30950.06.90253969
AACCCTC5800.00321235726.6349533
GTGTAGG41700.06.63484570
ATATCGG5900.00379142936.53078368
TGTAGGG34000.06.28456968
AGCGTCG74500.06.11120470
GGGAAAG23750.06.045885669
GAGTGTA12355.5379314E-75.95514170
GCGTCGT67650.05.953464570
GTAGGAT8255.2844686E-45.944417566
CGTCGTG61350.05.765626469
CGTGTAG38400.05.654577369
AGGGAAA33450.05.653759570
GAGCGTC81400.05.593178369
GTCGTGT55250.05.51476170
Produced by FastQC (version 0.11.5)