FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL11_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL11_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968678
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG2602.7481292E-816.16310570
TCGCCCC1600.00912455313.1194427
TGTGCCA5601.8189894E-1111.88180670
GTGTGCC16800.09.79727970
GTGCCTC12050.08.71868770
AGAGTGT33350.08.71563770
AGTGTGC19900.08.271070569
TCGTGTT10252.4920155E-107.858159570
ATTGCGA4050.00678040557.782036358
GAGTGTG27150.07.61025370
TGTGCCT14300.07.59176169
GCCTCTA9654.0541636E-87.259714668
AAGAGTG40300.07.125669569
GGGAAAG54500.06.74853768
TGCCTCT12502.237357E-96.72385170
CCTCTAT10903.7470818E-76.425698869
GGAAAGA64800.06.377109569
AAAGAGT54050.06.28482170
GAAAGAG61700.06.129929570
CGTGGAG7450.00100905516.11197766
Produced by FastQC (version 0.11.5)