FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_ColSPL10_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_ColSPL10_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015877
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCG7500.012.60841270
AGGCTAT23700.09.31000970
TGTAGGC38550.08.72177169
GAGTGTA65100.08.50029570
TAGGCTA28500.08.479341569
GGCTATA22250.08.34264470
AGTGTAG62000.08.24743870
GTAGGCT27250.08.22567169
AGAGTGT76500.07.46249569
GCTATAG18550.06.796987569
GTAGGCA9301.245071E-66.778716670
AAGAGTG87200.06.628623568
GTGTAGG231900.06.22234970
AGGGAAA159050.06.07761670
TGTAGGG178850.05.953090770
AAAGAGT122650.05.91099670
GGGAAAG121650.05.815634769
TAGGCGA7850.00188682555.801357368
TAGGGAA155750.05.779136769
GTAGGGA161350.05.77391770
Produced by FastQC (version 0.11.5)