FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_C24SPL16_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_C24SPL16_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906761
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC450.00430970931.11030236
CTCCCGG2000.002768274412.25841468
GAGCGGA3100.0068233289.03536157
TAGGGAA26500.06.7392370
CGTGTAT7301.0896682E-46.71569870
GTAGGGA26950.06.626701469
GTCGTGG6903.9898805E-46.598732567
AGTAGCT7055.224025E-46.4540731
GAGTGTT15254.0618033E-95.97020869
TGTAGGG29150.05.88740368
GCGTCGT55450.05.873170
AAGAGTG20301.8189894E-115.692510670
GTGTTAA13051.54382E-65.635010770
GTGTAGG31850.05.60824567
AGGGAAA30650.05.59824170
CGAGGAA8200.0032016715.548490534
CGTGTAG32400.05.51318966
CGTCGTG51500.05.50761769
AGCGTCG60100.05.418691669
TCGTGTA42700.05.412551469
Produced by FastQC (version 0.11.5)