FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_C24SPL14_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_C24SPL14_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492004
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCC500.007230009427.9849317
GCGGCTG1500.00595619513.99293727
GTGTGTC4753.932238E-58.84778169
GTCGTGG4300.00127704098.14475970
GTAGGGA18900.07.22685970
GAGTGTG9452.1645974E-77.04155570
CGTGTAG25300.06.92143770
AGTGTGT8954.928328E-66.652985668
TGTAGGG21400.06.382599469
GCGTCGT42450.06.35272170
GGGAAAG17207.2759576E-126.312188669
TCGTGTA32000.05.91004269
GTGTAGG23850.05.873789369
AGCGTCG46150.05.767513869
AGGGAAA22550.05.7464870
CGTCGTG39250.05.7106769
GTCGTGT34850.05.62771470
TAGGGAA19956.548362E-115.61763969
GAGCGTC50000.05.603595370
GGAAAGA22601.8189894E-125.578800770
Produced by FastQC (version 0.11.5)