FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_C24SPL11_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_C24SPL11_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148028
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79450.12922842901821527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG13700.023.52031570
TGTACGG23250.015.21508170
GTCCGGG9350.012.73629770
CCTGGTG25700.012.12921669
CGTCCGG8900.011.80611869
GTACGTG18000.011.48035670
TACGTCC64400.011.25797770
CTGGTGT32500.011.20794270
CGTCCTG41900.011.20125470
GTAGGGG24850.011.1346570
ACGTCCT42350.011.08223269
GCGTCGG14150.010.39605970
GTACGTC71700.09.965219569
CCGGGTG6402.242814E-99.8507369
TCGTGTT64000.09.79600370
CGGGTGT7951.0913936E-119.69240370
TACGGCC6857.0604074E-88.6922969
GTGTACG127600.08.59151269
GTCCTGG39550.08.32448369
TGTACGT104700.08.23087668
Produced by FastQC (version 0.11.5)