FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n02_AtTGA5_Nis_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n02_AtTGA5_Nis_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049726
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231600.45863874594383935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC8950.010.95706162
CGGCAGT8950.010.9495333
AACGGTG9400.010.80457260
CTCAACG9800.010.003612557
ACCGGCA9000.09.718760531
CGGCTTA10800.09.71837222
ACGGTGG10000.09.45529861
CCGGCTT10450.09.37371721
CCGGCAG9350.09.35691332
CCTCCGG10500.09.32908118
TCCGGCT10950.09.265182520
CACCGGC9550.09.15913330
GGCAGTC11600.09.05156534
GGCAACT11700.08.98100266
TCAACGG10550.08.96227858
TCACCGG10455.456968E-128.37039129
GCAGTCT13450.07.80663335
CAGTCTG13200.07.689412636
GGGTTCC12657.2759576E-127.471043647
CTCCGGC13303.6379788E-127.36506419
Produced by FastQC (version 0.11.5)